参考: 诱导性多能干细胞 Induced pluripotent stem cell Induced Pluripotent Stem Cells: Problems and Advantages when Applying them in Regenerative Medicine Induced pluripotency: history, mechanisms, and applications Targeting RNA foci in iPSC-derived motor neuron…
许多分析软件 : https://github.com/seandavi/awesome-single-cell#software-packages Smart-seq.CEL-seq.SCRB-seq和Drop-seq.Smart-seqSMART(Switching mechanism at 5’ end of the RNA transcript)是一个具有里程碑意义的重要技术.实际上,能够从单细胞生成全长cDNA的测序方案并不多,Smart-seq就是其中之一.对于等位基因特异性表达或者…
单细胞在脑科学方面的应用 Session 1: Deciphering the Cellular Landscape of the Brain Using Single Cell Transcriptomics Single cell/nucleus transcriptomics has emerged as a powerful approach to classify cell types and dynamic cell states in any multicellular organ…
Title: Multiclonal Invasion in Breast Tumors Identified by Topographic Single Cell Sequencing 课题的目的和意义: Ductal carcinoma in situ (DCIS,原位导管癌)是一种早期的乳腺癌,很少发展成invasive ductal carcinoma(IDC,浸润型导管癌).由于瘤内异质性和导管中肿瘤细胞数量低,因此很难刻画在侵袭期间的基因组进化过程.为了克服这个障碍,作者开发了To…
资源: sci-hub paper CellBench package - github CellBench_data - code for the paper 现在单细胞领域的突出问题就是工具过多,但缺乏gold-standard benchmark datasets,没有一定的标准来衡量工具的好坏. 另外人们也不容易根据自己的问题来选择合适的工具,所以保险起见,现在大家都只用最有名气的那几个工具:seurat.monocle.SC3等. 算法是无穷的,随便套几个方法就能组成一个分析方法,做生…
