转录组 测量单cell,可以认为是一种细胞.细胞株也认为来自同一个细胞. 使用两种方法,找mRNA(polyA)及rmRNA(ribo-minus),然后取交集. 转录组受实验影响,比如小片段没得到. 干细胞与诱导的干细胞序列相似的原因尚不清楚. 转录因子参与的转录过程,相同转录因子,老鼠可以转向干细胞,但是牛就不可以.说明真核细胞转录复杂性. 平衡与跌倒 干细胞的所有基因都在低表达状态.在分化过程中,受到能量分配影响,转录扩增细胞同时关闭一些gene和增多一些gene.HK维持表达,TS会关闭…

生物信息学 Cell theory:7个要点 All known living things are made up of one or more cells. All living cells arise from pre-existing cells by division. The cell is the fundamental unit of structure and function in all living organisms. The activity of an organi…

Downloading and installing the SRA Toolkit step1: 下载并安装SRAtoolkit    (Download the Toolkit from the SRA website) If you are using a web browser, the following page contains download links to the most current version of the toolkit for each of the sup…

单细胞在脑科学方面的应用 Session 1: Deciphering the Cellular Landscape of the Brain Using Single Cell Transcriptomics Single cell/nucleus transcriptomics has emerged as a powerful approach to classify cell types and dynamic cell states in any multicellular organ…

I'll share another review paper about Epigenetic Landscape, it comes from Nature Review, published in 2016. Transition states and cell fate decisions in epigenetic landscapes [1] In this paper, they suggest that there might be a fundamental flaw in t…

许多分析软件 : https://github.com/seandavi/awesome-single-cell#software-packages Smart-seq.CEL-seq.SCRB-seq和Drop-seq.Smart-seqSMART(Switching mechanism at 5’ end of the RNA transcript)是一个具有里程碑意义的重要技术.实际上,能够从单细胞生成全长cDNA的测序方案并不多,Smart-seq就是其中之一.对于等位基因特异性表达或者…

The normalization method described above aims to reduce the effect of technical factors in scRNA-seq data (primarily, depth) from downstream analyses. However, heterogeneity in cell cycle stage, particularly among mitotic cells transitioning between…

文献名:Quantitative mass spectrometry to interrogate proteomic heterogeneity in metastatic lung adenocarcinoma and validate a novel somatic mutation CDK12-G879V(利用定量质谱技术探究转移性肺腺瘤的蛋白质组异质性及验证新体细胞突变CDK12-G879V) 期刊名:Mol Cell Proteomics 发表时间:(2019年4月) IF:5.23…

Transcriptional benchmarking of in vitro cells to in vivo with single-cell rna-seq - 简介 Harnessing single-cell genomics to improve the physiological fidelity of organoid-derived cell types - 原文 目前发育生物学的实验基础有两类, 一是活体小鼠,作为模式动物:为了把小鼠上的研究造福于人类(诊断.筛查.药物开发…

Today, I'll share a review papers about Epigenetic Landscape, the Epigenetic Landscape is related to cell differentiation. It comes from Current Biology, published in 2012. Bistability, Bifurcations, and Waddington’s Epigenetic Landscape [1] This pap…