参考:高通量测序相关名词 Isotig 指在转录组de novo测序时,用454平台测序完成后组装出的结果,一个isotig可视为一个转录本. Isogroup 指转录组de novo测序中,用454平台测序完成后组装出的结果获得的可聚类到同一个基因的转录本群. alternative splicing:可变剪切 gene loci:基因座(wiki), 转录表达谱:又叫基因表达谱,翻译表达谱的话,就是蛋白表达谱. 表达谱差异分析(differential expression profilin…

可变剪接(alternative splicing),在真核生物中是一种非常基本的生物学事件.即基因转录后,先产生初始RNA或称作RNA前体,然后再通过可变剪接方式,选择性的把不同的外显子进行重连,从而产生不同的剪接异构体(isoform).这种方式,使得一个基因可产生多个不同的转录本,这些转录本分别在细胞/个体分化发育的不同阶段,在不同的组织中有各自特异的表达和功能,从而极大地丰富了编码RNA和非编码RNA种类和数量,进而增加了转录组和蛋白质组的复杂性. 可变剪接主要有以下五种常见的形式: 1…

全基因组关联分析除了找到显著的关联位点,我们还可以做基因优化.geneset富集分析.组织富集分析,下面具体讲一讲怎么利用GWAS的summary数据做这个分析. summary数据就是关联分析的结果文件 1 软件安装前请确保需要满足的系统环境 1.1 支持Mac OS X 或者 UNIX, 不支持windows系统 1.2 Java SE 6(或者更高),没有安装Java请自行安装 1.3 需要PIP 怎么确定系统有没有安装PIP呢,输入命令which pip,如果没有路径弹出,说明没有PIP…

做基因组注释 先用augustus训练,然后再用maker做基因注释 augustus提供一些训练好的,如果有和你的物种非常接近的,直接用提供的,没有的话再自己训练. 网址: http://bioinf.uni-greifswald.de/augustus/ 老版本下载: http://bioinf.uni-greifswald.de/augustus/binaries/old/ 最后选择下载2.7的 新版本3.2的实在是装不上 太麻烦了!!!!! 下载好后,解压,cd src, sudo ma…

转载: https://github.com/twbattaglia/RNAseq-workflow Introduction RNAseq is becoming the one of the most prominent methods for measuring celluar responses. Not only does RNAseq have the ability to analyze differences in gene expression between samples,…

Human Gene Functions Time Limit: 1000MS Memory Limit: 10000K Total Submissions: 18053 Accepted: 10046 Description It is well known that a human gene can be considered as a sequence, consisting of four nucleotides, which are simply denoted by four let…

题目地址:http://poj.org/problem?id=1080 Description It is well known that a human gene can be considered as a sequence, consisting of four nucleotides, which are simply denoted by four letters, A, C, G, and T. Biologists have been interested in identifyi…

Human Gene Functions Time Limit: 1000MS   Memory Limit: 10000K Total Submissions: 17206   Accepted: 9568 Description It is well known that a human gene can be considered as a sequence, consisting of four nucleotides, which are simply denoted by four…

Problem Description It is well known that a human gene can be considered as a sequence, consisting of four nucleotides, which are simply denoted by four letters, A, C, G, and T. Biologists have been interested in identifying human genes and  determin…

题目描述 As a gene engineer of a gene engineering project, Enigma encountered a puzzle about gene recombination. It is well known that a gene can be considered as a sequence, consisting of four nucleotides, which are simply denoted by four letters, A, C,…