折腾这么多都是白瞎,STAR就有输出没有别对上的pair-end reads的功能 参见:How To Filter Mapped Reads With Samtools I had the same issue but with Paired End Reads, and I solved using samtools and bamToFastq. You can find bamToFastq here: https://code.google.com/p/hydra-sv/ If you
文献:Sahraeian S M E, Mohiyuddin M, Sebra R, et al. Gaining comprehensive biological insight into the transcriptome by performing a broad-spectrum RNA-seq analysis[J]. Nature Communications, 2017, 8(1):59. 这是一篇在NC上发表的使用RNAseq工具对比的一篇文献,解读这篇文献对我们使用RNAseq
Use an append-only store to record the full series of events that describe actions taken on data in a domain, rather than storing just the current state, so that the store can be used to materialize the domain objects. This pattern can simplify tasks
Flask-RESTful是flask的扩展,增加了对快速构建REST API的支持. Flask-RESTful通过最少的设置鼓励最佳的实践. pip install flask-restfulFlask-RESTful需要Python版本2.7,3.4,3.5,3.6或3.7 资源和路由 from flask-restful import Resource,Api app = Flask(name) api = Api(app) class TodoSimple(Resource): pas