http://icb.med.cornell.edu/wiki/index.php/Elementolab/ChIPseeqer_Tutorial [怪毛匠子 整理] ChIP-seq[核心分析 下游分析] Core Analysis : Peak detection: Split raw data then run ChIPseeqer Core Analysis : Quality Control: QC analysis for the raw reads (after Split raw
这是一个进化学上的概念,基因组的序列是不断进化而来的,根据45个脊椎动物的基因组序列,通过多重比对,我们就可以知道人类基因组上每个位置的保守性,一些高度保守的区域可以做非常有意思的下游分析. This directory contains compressed phastCons scores for multiple alignments of 45 vertebrate genomes to the human genome, plus an alternate set of scores
总是跑数据,却对数据一无所知,这说不过去吧. 看几篇文章吧 Sequencing depth and coverage: key considerations in genomic analyses(只讲二代) Assembly of large genomes using second-generation sequencing(参考文献) Identification of optimum sequencing depth especially for de novo genome asse